Year: 2018 | Month: August | Volume 8 | Issue 4
Ameliorating Effect of Standard Treatment on Cerulein-Induced Acute Pancreatitis in Rat Model
Abstract:
An experiment was designed on diagnostic biomarkers and effect of standard therapy followed during acute pancreatitis in rat model. Rats were divided in three groups, A (Control), B (acute pancreatitis) and C (treatment) groups, 12 rats in each. Supra-maximal dose of cerulein (50 μg/kg b wt) was given i.p. at hourly interval for 7 h in groups B and C. Group A was treated with 0.25 ml of NSS at same interval. Treatment and sampling started after 12 h of last injection of cerulein. Group C rats were treated by antibiotic, anti-inflammatory and vit-E. Hematological and biochemical estimations were carried out at 12, 24, 60 and 156 h of last dose of cerulein. Hematological parameters found to increase significantly in group B and C than A and significant reduction noted in group C after treatment. Serum lipase and amylase were considered as accurate biomarkers and their values were found to increase several times in induction groups and became almost normal at 156 h of sampling in C group. Significant reduction in AST and ALT values noticed in treatment group. BUN and creatinine became normal in Group C at 156 and 60 h, respectively. Serum calcium became normal at 60 h. It was concluded that significant increase in serum lipase, amylase, AST, ALT, BUN, creatinine and CK-MB had diagnostic significance of acute pancreatitis and their values became normal in treatment group earlier and faster than group AP showing that standard treatment had significant role in acute pancreatitis.
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